GeneTex
United States (US)

ETS1 antibody

Comparison Orthogonal
GTX100639_42893_20170825_WB_Fraction_competitor_watermark_19011506_506.jpg
GTX100639_42893_20170622_WB_19011506_611.jpg

Cat No. GTX100639

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Jurkat , Jurkat nuclear extract

Predict Reactivity

Rat, Rabbit(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ETS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ets Proto-Oncogene 1, Transcription Factor,Ets-1,Ewsr2,C-Ets-1,P54,Ets1

Cellular Localization

Nucleus

Background

ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX100639_42893_20170825_WB_Fraction_competitor_watermark_19011506_506.jpg

GTX100639 WB Image

Comparison Orthogonal

Jurkat whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with ETS1 antibody [N3C1], Internal (GTX100639) diluted at 1:500 and competitor's antibody (sc-350) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX100639_42893_20170622_WB_19011506_611.jpg

GTX100639 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ETS1 antibody [N3C1], Internal (GTX100639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf