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ETS1 antibody

Anti-ETS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52308
Anti-ETS1 antibody used in Western Blot (WB). GTX52308
Anti-ETS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX52308

Cat. No. GTX52308

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ETS1 (11-60).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ETS protooncogene 1, transcription factor , ETS1 , EWSR2 , cets1 , p54 , v-ets avian erythroblastosis virus E26 oncogene homolog 1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-ETS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52308

GTX52308 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX52308 ETS1 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ETS1 antibody used in Western Blot (WB). GTX52308

GTX52308 WB Image

WB analysis of HeLa cells treated with 20% Serum (15mins) lysate using GTX52308 ETS1 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ETS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX52308

GTX52308 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX52308 ETS1 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399