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ETS2 antibody

Orthogonal
Anti-ETS2 antibody used in Immunoprecipitation (IP). GTX104527
Anti-ETS2 antibody used in Western Blot (WB). GTX104527
Anti-ETS2 antibody used in ChIP assay (ChIP assay). GTX104527
Anti-ETS2 antibody used in Western Blot (WB). GTX104527
Anti-ETS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104527
Anti-ETS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104527
Anti-ETS2 antibody used in ChIP assay (ChIP assay). GTX104527
Anti-ETS2 antibody used in Western Blot (WB). GTX104527

Cat. No. GTX104527

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ETS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179034

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ETS proto-oncogene 2, transcription factor , ETS2IT1

Cellular Localization

Nucleus

Background

ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ETS2 antibody used in Immunoprecipitation (IP). GTX104527

GTX104527 IP Image

ETS2 antibody immunoprecipitates ETS2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ETS2 protein by 2 μg of ETS2 antibody (GTX104527) 7.5% SDS-PAGE The immunoprecipitated ETS2 protein was detected by ETS2 antibody (GTX104527) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ETS2 antibody used in Western Blot (WB). GTX104527

GTX104527 WB Image

Orthogonal

Untreated (–) and treated (+) Raw 264.7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETS2 antibody (GTX104527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ETS2 antibody used in ChIP assay (ChIP assay). GTX104527

GTX104527 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ETS2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PTHrP promoter.
ChIP experiment and primer designs are based on J Biol Chem. 2008 Aug 29;283(35):23567-80.

Anti-ETS2 antibody used in Western Blot (WB). GTX104527

GTX104527 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ETS2 antibody (GTX104527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ETS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104527

GTX104527 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ETS2(GTX104527) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-ETS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104527

GTX104527 IHC-P Image

ETS2 antibody detects ETS2 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
ETS2 antibody (GTX104527) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ETS2 antibody used in ChIP assay (ChIP assay). GTX104527

GTX104527 ChIP assay Image

The data was published in the journal Oncotarget in 2017. PMID: 28978027

Anti-ETS2 antibody used in Western Blot (WB). GTX104527

GTX104527 WB Image

The data was published in the journal PLoS Genet in 2019. PMID: 31306413

REFERENCE

Application Reference

Kishimoto Y et al. Sci Rep 2021; 11 (1) : 8297 A novel tissue specific alternative splicing variant mitigates phenotypes in?Ets2?frame-shift mutant models.
Application : WB
Reactivity : Mouse
Gluck C et al. PLoS Genet 2019; 15 (7) : e1008250 Molecular dissection of the oncogenic role of ETS1 in the mesenchymal subtypes of head and neck squamous cell carcinoma.
Application : WB
Reactivity : Human
Helbig S et al. Oncotarget 2017; 8 (40) : 67203-67217 Functional dissection of breast cancer risk-associated TERT promoter variants.
Yuan J et al. Cancer Res 2017; (Epub) Super-enhancers promote transcriptional dysregulation in nasopharyngeal carcinoma.
Application : IHC-P
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169