GeneTex
United States (US)

EVI1 antibody, Internal

GTX88191_2505_WB_18121411_595.jpg

Cat No. GTX88191

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88191-PEP).

Specificity/Sensitivity

This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DKESLHSTSH, from the internal region of the protein sequence according to NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mds1 And Evi1 Complex Locus,Aml1-Evi-1,Evi1,Kmt8E,Mds1,Mds1-Evi1,Prdm3,Rusat2,Mecom

Background

The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Research Area

DATA IMAGES
GTX88191_2505_WB_18121411_595.jpg

GTX88191 WB Image

WB analysis of A549 lysate using GTX88191 EVI1 antibody, Internal.
Dilution : 2µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329