GeneTex
United States (US)

EWSR1 antibody, Internal

GTX88471_2291_WB_18122102_916.jpg
GTX88471_949_IHC-P_18122102_976.jpg
GTX88471_950_IHC-P_18122102_905.jpg

Cat No. GTX88471

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88471-PEP).

Specificity/Sensitivity

This antibody is expected to recognize all five reported isoforms (NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1).

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TSYDQSSYSQQNTYG, from the internal region of the protein sequence according to NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ews Rna Binding Protein 1,Ews,Ews-Fli1,Bk984G1.4,Ewsr1

Cellular Localization

Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.

Background

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]

Database

Research Area

DATA IMAGES
GTX88471_2291_WB_18122102_916.jpg

GTX88471 WB Image

WB analysis of HeLa nuclear lysate using GTX88471 EWSR1 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88471_949_IHC-P_18122102_976.jpg

GTX88471 IHC-P Image

IHC-P analysis of human small intestine using GTX88471 EWSR1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88471_950_IHC-P_18122102_905.jpg

GTX88471 IHC-P Image

IHC-P analysis of human uterus using GTX88471 EWSR1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

Package List Price ($)
$ 329