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EWSR1 antibody

Anti-EWSR1 antibody used in Western Blot (WB). GTX114069
Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069
Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069
Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069
Anti-EWSR1 antibody used in Western Blot (WB). GTX114069
Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069
Anti-EWSR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114069
Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069

Cat. No. GTX114069

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EWSR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Observed MW

96 kDa. The observed M.W. is based on the following publication. PMID: 20665663

Positive Control

293T , HeLa , HepG2

Predict Reactivity

Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EWSR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620780

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EWS RNA binding protein 1 , EWS , EWS-FLI1 , bK984G1.4

Cellular Localization

Nucleus , Cytoplasm , Cell membrane

Background

This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EWSR1 antibody used in Western Blot (WB). GTX114069

GTX114069 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EWSR1 antibody (GTX114069) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069

GTX114069 IHC-P Image

EWSR1 antibody detects EWSR1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EWSR1 antibody (GTX114069) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069

GTX114069 IHC-P Image

EWSR1 antibody detects EWSR1 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
EWSR1 antibody (GTX114069) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069

GTX114069 IHC-P Image

EWSR1 antibody detects EWSR1 protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse small intestine. EWSR1 antibody (GTX114069) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EWSR1 antibody used in Western Blot (WB). GTX114069

GTX114069 WB Image

EWSR1 antibody detects EWSR1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EWSR1 antibody (GTX114069) diluted at a dilution of 1:1000.
Observed molecular weight of ~96 kDa based on published findings [see: J Cell Biochem. 2010 November 1; 111(4): . doi:10.1002/jcb.22782.]

Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069

GTX114069 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cal27 xenograft, using EWSR1(GTX114069) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EWSR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114069

GTX114069 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EWSR1(GTX114069) antibody at 1:500 dilution.

Anti-EWSR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114069

GTX114069 IHC-P Image

EWSR1 antibody detects EWSR1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
EWSR1 antibody (GTX114069) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Tulpule A et al. Res Sq 2023; FET fusion oncoproteins disrupt physiologic DNA repair networks and induce ATR synthetic lethality in cancer.
Menon S et al. bioRxiv 2023; FET fusion oncoproteins disrupt physiologic DNA repair networks in cancer.
Kong X et al. J Cell Sci 2018; 131 (23) Biphasic recruitment of TRF2 to DNA damage sites promotes non-sister chromatid homologous recombination repair.
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169