APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5-1.5μg/ml |
5-10μg/ml |
Note :
IHC-P
Human Adrenal Gland shows staining of cytoplasm membranes and cytoplasm of secretory cells in the medulla .
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88750-PEP).
Product Note
This antibody is expected to recognise all five reported isoforms (NP_001013861.1; NP_056034.2; NP_001138769.1; NP_001138771.1; NP_001138770.1).
Predict Reactivity
Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-DNIKNDPDKEYN, from the internal region of the protein sequence according to NP_001013861.1; NP_056034.2; NP_001138769.1; NP_001138771.1; NP_001138770.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
exocyst complex component 7 , 2-5-3p , BLOM4 , EX070 , EXO70 , EXOC1 , Exo70p , YJL085W
Cellular Localization
Cytoplasm, cytosol , Cell membrane
Background
The protein encoded by this gene is a component of the exocyst complex. The exocyst complex plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. The encoded protein is required for assembly of the exocyst complex and docking of the complex to the plasma membrane. The encoded protein may also play a role in pre-mRNA splicing through interactions with pre-mRNA-processing factor 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Nov 2011]
Database
Research Area
DATA IMAGES
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GTX88750 WB Image
WB analysis of human lung lysate using GTX88750 EXOC7 antibody, Internal. Dilution : 0.5μg/ml Loading : 35μg protein in RIPA buffer
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GTX88750 IHC-P Image
IHC-P analysis of human pancreas using GTX88750 EXOC7 antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 0.5μg/ml
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REFERENCE
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REVIEW
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