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EXOC7 antibody [N1C1]

Anti-EXOC7 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112132
Anti-EXOC7 antibody [N1C1] used in Western Blot (WB). GTX112132

Cat No. GTX112132

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all EXOC7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Molt-4

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EXOC7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950236

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

exocyst complex component 7 , 2-5-3p , BLOM4 , EX070 , EXO70 , EXOC1 , Exo70p , YJL085W

Cellular Localization

Cytoplasm , cytosol , Cell membrane; Peripheral membrane protein

Background

EXOC7 is a component of the exocyst, which is an evolutionarily conserved octameric protein complex essential for exocytosis. The exocyst targets secretory vesicles at specific domains of the plasma membrane for cell surface expansion and protein secretion (Zuo et al., 2006 [PubMed 17086175]).[supplied by OMIM]

Database

DATA IMAGES

Anti-EXOC7 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112132

GTX112132 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using EXOC7(GTX112132) antibody at 1:200 dilution.

Anti-EXOC7 antibody [N1C1] used in Western Blot (WB). GTX112132

GTX112132 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX112132 diluted at 1:1000

REFERENCE

Application Reference

Chernov AV et al. PLoS One 2015; 10 (11) : e0142529 Depletion of CG-Specific Methylation in Mycoplasma hyorhinis Genomic DNA after Host Cell Invasion.
Application : ICC/IF
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169