GeneTex
United States (US)

EXOSC10 antibody

Overexpression
GTX107856_40044_IP_19011507_334.jpg
GTX107856_IHC_19011507_900.jpg
GTX107856_40044_20180720_WB_B_19011507_165.jpg
GTX107856_WB_19011507_244.jpg

Cat No. GTX107856

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

293T , EXOSC10-transfected 293T

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EXOSC10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Exosome Component 10,Pm-Scl,Pm/Scl-100,Pmscl,Pmscl2,Rrp6,Rrp6P,P2,P3,P4,Exosc10

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Database

DATA IMAGES
GTX107856_40044_IP_19011507_334.jpg

GTX107856 IP Image

EXOSC10 antibody immunoprecipitates EXOSC 10 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of EXOSC 10 protein by 2.5 μg of EXOSC 10 antibody (GTX107856) 7.5% SDS-PAGE The immunoprecipitated EXOSC 10 protein was detected by EXOSC10 antibody (GTX107856) diluted at 1:1000.

GTX107856_IHC_19011507_900.jpg

GTX107856 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Exosome component 10 (GTX107856) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107856_40044_20180720_WB_B_19011507_165.jpg

GTX107856 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody (GTX107856) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107856_WB_19011507_244.jpg

GTX107856 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX107856 diluted at 1:3000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169