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EXOSC10 antibody

Overexpression
Anti-EXOSC10 antibody used in Immunoprecipitation (IP). GTX107856
Anti-EXOSC10 antibody used in Western Blot (WB). GTX107856
Anti-EXOSC10 antibody used in Western Blot (WB). GTX107856
Anti-EXOSC10 antibody used in IHC (Paraffin sections) (IHC-P). GTX107856

Cat. No. GTX107856

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EXOSC10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

293T , EXOSC10-transfected 293T

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EXOSC10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950238

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

exosome component 10 , PM-Scl , PM/Scl-100 , PMSCL , PMSCL2 , RRP6 , Rrp6p , p2 , p3 , p4

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Database

Research Area

DATA IMAGES

Anti-EXOSC10 antibody used in Immunoprecipitation (IP). GTX107856

GTX107856 IP Image

EXOSC10 antibody immunoprecipitates EXOSC 10 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of EXOSC 10 protein by 2.5 μg of EXOSC 10 antibody (GTX107856) 7.5% SDS-PAGE The immunoprecipitated EXOSC 10 protein was detected by EXOSC10 antibody (GTX107856) diluted at 1:1000.

Anti-EXOSC10 antibody used in Western Blot (WB). GTX107856

GTX107856 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody (GTX107856) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EXOSC10 antibody used in Western Blot (WB). GTX107856

GTX107856 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX107856 diluted at 1:3000

Anti-EXOSC10 antibody used in IHC (Paraffin sections) (IHC-P). GTX107856

GTX107856 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Exosome component 10 (GTX107856) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169