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EZH1 antibody, N-term

Anti-EZH1 antibody, N-term used in Western Blot (WB). GTX89888

Cat. No. GTX89888

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all EZH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-6μg/ml
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89888-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence EIPNPPTSKCITY, from the N Terminus of the protein sequence according to NP_001982.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enhancer of zeste 1 polycomb repressive complex 2 subunit , KMT6B

Cellular Localization

Nucleus

Background

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-EZH1 antibody, N-term used in Western Blot (WB). GTX89888

GTX89888 WB Image

WB analysis of Molt-4 cell lysate using GTX89888 EZH1 antibody, N-term.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399