GeneTex
United States (US)

EZH1 antibody [N1], N-term

GTX108013_WB_19011507_175.jpg
GTX108013_IHC_19011507_326.jpg
GTX108013_39721_IFA_19011507_300.jpg

Cat No. GTX108013

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human EZH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Enhancer Of Zeste 1 Polycomb Repressive Complex 2 Subunit,Kmt6B,Ezh1

Cellular Localization

Nucleus

Background

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX108013_WB_19011507_175.jpg

GTX108013 WB Image

Sample(30 µg of whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX108013 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108013_IHC_19011507_326.jpg

GTX108013 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using EZH1(GTX108013) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108013_39721_IFA_19011507_300.jpg

GTX108013 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EZH1(GTX108013) antibody at 1:1000 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169