Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EZH1(GTX108013) antibody at 1:1000 dilution.

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using EZH1(GTX108013) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EZH1 antibody [N1], N-term (GTX108013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.