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EZH2 antibody - ChIP grade

Anti-EZH2 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60811
Anti-EZH2 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60811
Anti-EZH2 antibody - ChIP grade used in Western Blot (WB). GTX60811
Anti-EZH2 antibody - ChIP grade used in Western Blot (WB). GTX60811
Anti-EZH2 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60811

Cat. No. GTX60811

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ChIP assay, RIP

Reactivity

 Human, Mouse
Package
50 μg ($429)
See all EZH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
ChIP assay Assay dependent
RIP Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

The N-terminus (aa1-343) of the mouse EZH2 protein (Enhancer of zeste homolog 2).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enhancer of zeste 2 polycomb repressive complex 2 subunit , Enx-1 , Enx1h , KMT6 , mKIAA4065

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-EZH2 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60811

GTX60811 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60811 EZH2 antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000

Anti-EZH2 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60811

GTX60811 ChIP assay Image

ChIP analysis of sheared chromatin from 4x10⁶ K562 cells using GTX60811 EZH2 antibody - ChIP grade. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-EZH2 antibody - ChIP grade used in Western Blot (WB). GTX60811

GTX60811 WB Image

WB analysis of nuclear extracts (40 μg) from HeLa cells using GTX60811 EZH2 antibody - ChIP grade.
Dilution : 1:1,000

Anti-EZH2 antibody - ChIP grade used in Western Blot (WB). GTX60811

GTX60811 WB Image

WB analysis of whole cell extracts (40 μg) from HeLa cells transfected with EZH2 siRNA (lane 2) and from an untransfected control (lane 1) using GTX60811 EZH2 antibody - ChIP grade.
Dilution : 1:1,000

Anti-EZH2 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60811

GTX60811 ChIP assay Image

ChIP analysis of sheared chromatin from 4 million K562 cells using GTX60811 EZH2 antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure 2A and B, respectively), and in two genomic regions containing the MYT1 gene on chromosome 20 and the HOX cluster on chromosome 7 (figure 2C and D).
Antibody amount : 2μg

REFERENCE

Application Reference

D Gu et al. Immunobiology 2024; : 152796 Transcription suppression of GABARAP mediated by lncRNA XIST-EZH2 interaction triggers caspase-11-dependent inflammatory injury in ulcerative colitis
Application : RIP
Submit a Reference

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Package List Price ($)
$ 429