EZH2 antibody - ChIP grade

ChIP analysis of sheared chromatin from 4 million K562 cells using GTX60811 EZH2 antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure 2A and B, respectively), and in two genomic regions containing the MYT1 gene on chromosome 20 and the HOX cluster on chromosome 7 (figure 2C and D).
Antibody amount : 2μg

WB analysis of whole cell extracts (40 μg) from HeLa cells transfected with EZH2 siRNA (lane 2) and from an untransfected control (lane 1) using GTX60811 EZH2 antibody - ChIP grade.
Dilution : 1:1,000

WB analysis of nuclear extracts (40 μg) from HeLa cells using GTX60811 EZH2 antibody - ChIP grade.
Dilution : 1:1,000

ChIP analysis of sheared chromatin from 4x10⁶ K562 cells using GTX60811 EZH2 antibody - ChIP grade. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60811 EZH2 antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000