GeneTex
  • Country / Location Selection

United States (US)

EZH2 antibody

Anti-EZH2 antibody used in ChIP assay (ChIP assay). GTX110384
Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110384
Anti-EZH2 antibody used in Western Blot (WB). GTX110384

Cat No. GTX110384

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ChIP assay

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

H1299 , HCT116 , MCF-7

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EZH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enhancer of zeste 2 polycomb repressive complex 2 subunit , ENX-1 , ENX1 , EZH2b , KMT6 , KMT6A , WVS , WVS2

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EZH2 antibody used in ChIP assay (ChIP assay). GTX110384

GTX110384 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-EZH2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.

Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110384

GTX110384 ICC/IF Image

EZH2 antibody detects EZH2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EZH2 protein stained by EZH2 antibody (GTX110384) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EZH2 antibody used in Western Blot (WB). GTX110384

GTX110384 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
B: HCT116
C: MCF-7
7.5% SDS PAGE
GTX110384 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

EZH2 antibody Cat No. GTX110384

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169