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EZH2 antibody

Anti-EZH2 antibody used in Western Blot (WB). GTX110384
Anti-EZH2 antibody used in ChIP assay (ChIP assay). GTX110384
Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110384
Anti-EZH2 antibody used in Western Blot (WB). GTX110384

Cat. No. GTX110384

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ChIP assay

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all EZH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

H1299 , HCT116 , MCF-7

Predict Reactivity

Mouse, Zebrafish, Bovine, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EZH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885283

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enhancer of zeste 2 polycomb repressive complex 2 subunit , ENX-1 , ENX1 , EZH2b , KMT6 , KMT6A , WVS , WVS2

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EZH2 antibody used in Western Blot (WB). GTX110384

GTX110384 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EZH2 antibody (GTX110384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EZH2 antibody used in ChIP assay (ChIP assay). GTX110384

GTX110384 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-EZH2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.

Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110384

GTX110384 ICC/IF Image

EZH2 antibody detects EZH2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EZH2 protein stained by EZH2 antibody (GTX110384) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EZH2 antibody used in Western Blot (WB). GTX110384

GTX110384 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
B: HCT116
C: MCF-7
7.5% SDS PAGE
GTX110384 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Qian X et al. Bioengineered 2022; 13 (5) : 12583-12597 EZH2 enhances proliferation and migration of trophoblast cell lines by blocking GADD45A-mediated p38/MAPK signaling pathway.
Liao X et al. Mol Med Rep 2020; 22 (2) : 803-809 Effects of miR?195?5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2.
Application : WB
Reactivity : Human
Blin G et al. Nutrients 2020; 12 (1) Maternal Exposure to High-Fat Diet Induces Long-Term Derepressive Chromatin Marks in the Heart.
Application : WB
Reactivity : Rat
Yang PM et al. Am J Cancer Res 2019; 9 (10) : 2120-2139 p38帢/S1P/SREBP2 activation by the SAM-competitive EZH2 inhibitor GSK343 limits its anticancer activity but creates a druggable vulnerability in hepatocellular carcinoma.
Application : WB
Reactivity : Human
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REVIEW

EZH2 antibody Cat. No. GTX110384

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-EZH2 antibody used in Western Blot (WB). GTX110384
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169