APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 10 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)
Product Note
Multiple isoforms of EZH2 are known to exist. EZH2 antibody is predicted to not cross-react with EZH1.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
EZH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human EZH2.The immunogen is located within amino acids 190 - 240 of EZH2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
enhancer of zeste 2 polycomb repressive complex 2 subunit , ENX-1 , ENX1 , EZH2b , KMT6 , KMT6A , WVS , WVS2
Cellular Localization
Nucleus
Background
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
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GTX31435 ICC/IF Image
ICC/IF analysis of 293 cells using GTX31435 EZH2 antibody.
Working concentration : 20 μg/ml
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GTX31435 WB Image
WB analysis of 293 cell lysate using GTX31435 EZH2 antibody.
Working concentration : 1 μg/ml
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GTX31435 ICC/IF Image
ICC/IF analysis of 293 cells using GTX31435 EZH2 antibody.
Working concentration : 10 μg/ml
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REFERENCE
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REVIEW
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