GeneTex
United States (US)

EZH2 antibody

Anti-EZH2 antibody used in Western Blot (WB). GTX31435
Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31435
Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31435

Cat No. GTX31435

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)

Specificity/Sensitivity

Multiple isoforms of EZH2 are known to exist. EZH2 antibody is predicted to not cross-react with EZH1.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

EZH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human EZH2.The immunogen is located within amino acids 190 - 240 of EZH2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit,Enx-1,Enx1,Ezh2B,Kmt6,Kmt6A,Wvs,Wvs2,Ezh2

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES
Anti-EZH2 antibody used in Western Blot (WB). GTX31435

GTX31435 WB Image

WB analysis of 293 cell lysate using GTX31435 EZH2 antibody.
Working concentration : 1 μg/ml

Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31435

GTX31435 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31435 EZH2 antibody.
Working concentration : 10 μg/ml

Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31435

GTX31435 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31435 EZH2 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329