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EZH2 antibody

Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82503
Anti-EZH2 antibody used in Western Blot (WB). GTX82503
Anti-EZH2 antibody used in Western Blot (WB). GTX82503
Anti-EZH2 antibody used in Flow cytometry (FACS). GTX82503
Anti-EZH2 antibody used in Western Blot (WB). GTX82503
Anti-EZH2 antibody used in Western Blot (WB). GTX82503

Cat. No. GTX82503

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all EZH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:25
IHC-P Assay dependent
FACS 1:25
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This EZH2 antibody is generated from rabbits immunized with a recombinant fragment (N-term) protein from human EZH2.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11167802

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enhancer of zeste 2 polycomb repressive complex 2 subunit , ENX-1 , ENX1 , EZH2b , KMT6 , KMT6A , WVS , WVS2

Cellular Localization

Nucleus

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-EZH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82503

GTX82503 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82503 EZH2 antibody.
Green : EZH2
Red : Actin filament
Fixation : 4% paraformaldehyde (PFA)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25

Anti-EZH2 antibody used in Western Blot (WB). GTX82503

GTX82503 WB Image

WB analysis of various samples using GTX82503 EZH2 antibody.
Lane 1: mouse testis lysates
Lane 2: MDA-MB-231 whole cell lysates
Loading : 20 μg per lane
Dilution : 1:2000

Anti-EZH2 antibody used in Western Blot (WB). GTX82503

GTX82503 WB Image

WB analysis of Jurkat whole cell lysate using GTX82503 EZH2 antibody.
Loading : 20 μg per lane
Dilution : 1:2000

Anti-EZH2 antibody used in Flow cytometry (FACS). GTX82503

GTX82503 FACS Image

FACS analysis of HeLa cells using GTX82503 EZH2 antibody.
Green : EZH2
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

Anti-EZH2 antibody used in Western Blot (WB). GTX82503

GTX82503 WB Image

WB analysis of various samples using GTX82503 EZH2 antibody.
Lane 1: HeLa whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: Neuro-2a whole cell lysate
Lane 5: T47D whole cell lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-EZH2 antibody used in Western Blot (WB). GTX82503

GTX82503 WB Image

WB analysis of MDA-MB-468 whole cell lysate using GTX82503 EZH2 antibody.
Loading : 20 μg per lane
Dilution : 1:500

REFERENCE

Application Reference

Qian X et al. Bioengineered 2022; 13 (5) : 12583-12597 EZH2 enhances proliferation and migration of trophoblast cell lines by blocking GADD45A-mediated p38/MAPK signaling pathway.
Fathy A et al. Turk Patoloji Derg 2018; (Epub) EZH2, Endothelin-1, and CD34 as Biomarkers of Aggressive Cervical Squamous Cell Carcinoma: An Immunohistochemical Study.
Application : IHC-P
Reactivity : Human
Hsieh YY et al. Am J Cancer Res 2016; 6 (8) : 1661-80 EZH2 inhibitors transcriptionally upregulate cytotoxic autophagy and cytoprotective unfolded protein response in human colorectal cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399