GeneTex
United States (US)

EasyBlot anti Goat IgG (HRP)

GTX628547-01_WB_G_comparison_18121411_722.jpg
GTX628547-01_41215_WB_18121411_832.jpg

Cat No. GTX628547-01

Application WB
PRODUCT

Product Description

EasyBlot anti-Goat IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of Goat IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments.

Note: 1.Suggested dilution is 1:1000. Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples.
2.Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to rabbit IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Rabbit IgG (Optimized for Prot A/G) (GTXGTX225856-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Rabbit IgG Agarose beads (GTX27019) instead of Protein A/G.
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA, 40% Glycerol (pH7). 0.005% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Goat

Target Isotype

IgG

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
GTX628547-01_WB_G_comparison_18121411_722.jpg

GTX628547-01 WB Image

EasyBlot anti-Goat IgG (HRP)
Comparison of western blot analysis with traditional secondary antibody (GTX228416-01 diluted at 1:5000) (1) and EasyBlot anti-Goat IgG kit (GTX628547-01 diluted at 1:2000) (2).
A. 0.2 µg native goat IgG
B. 0.1 µg native goat IgG
C. 1 µg denatured goat IgG
D. 2 µg denatured goat IgG
E. 4 µg denatured goat IgG
F. 8 µg denatured goat IgG

GTX628547-01_41215_WB_18121411_832.jpg

GTX628547-01 WB Image

GST antibody immunoprecipitates GST-fused protein in IP experiments.
IP Sample: GST-fused protein expressed E. coli lysate
A. total lysate
B. Control with 1 μg of preimmune goat IgG
C. Immunoprecipitation of GST-fused protein by 1 μg of GST antibody (GTX26633)
12% SDS-PAGE
The immunoprecipitated GST-fuesd protein was detected by GST antibody (GTX26633) diluted at 1:10000.
EasyBlot anti-goat IgG (GTX628547-01) was used as a secondary reagent.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 199
$ 89