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EasyBlot anti Goat IgG (HRP)

EasyBlot anti Goat IgG (HRP). GTX628547-01
EasyBlot anti Goat IgG (HRP). GTX628547-01

Cat No. GTX628547-01

Application

WB

Reactivity

Goat
Package
250 μl ($199),
50 μl ($89)
PRODUCT

Summary

EasyBlot anti-Goat IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of Goat IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to goat IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-goat IgG (Optimized for Prot A/G) (GTX228910-01) or EasyBloker (GTX425858) to minimize protein A/G contamination.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000

Note :

WB
Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA, 40% Glycerol. 0.005% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Goat

Target Isotype

IgG

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

EasyBlot anti Goat IgG (HRP). GTX628547-01

GTX628547-01 WB Image

EasyBlot anti-Goat IgG (HRP)
Comparison of western blot analysis with traditional secondary antibody (GTX228416-01 diluted at 1:5000) (1) and EasyBlot anti-Goat IgG kit (GTX628547-01 diluted at 1:2000) (2).
A. 0.2 μg native goat IgG
B. 0.1 μg native goat IgG
C. 1 μg denatured goat IgG
D. 2 μg denatured goat IgG
E. 4 μg denatured goat IgG
F. 8 μg denatured goat IgG

EasyBlot anti Goat IgG (HRP). GTX628547-01

GTX628547-01 WB Image

GST antibody immunoprecipitates GST-fused protein in IP experiments.
IP Sample: GST-fused protein expressed E. coli lysate
A. total lysate
B. Control with 1 μg of preimmune goat IgG
C. Immunoprecipitation of GST-fused protein by 1 μg of GST antibody (GTX26633)
12% SDS-PAGE
The immunoprecipitated GST-fuesd protein was detected by GST antibody (GTX26633) diluted at 1:10000.
EasyBlot anti-goat IgG (GTX628547-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 199
$ 89