Summary
EasyBlot anti-Mouse IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of mouse IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP).
The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex’s EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.
Content
EasyBlot Kit Package |
Content |
Package |
Storage Temp |
GTX225857-01 (250μl) |
GTX221667-01 EasyBlot anti-Mouse IgG (HRP) |
250μl |
-20 ºC |
GTX425858 EasyBlocker |
60g |
4 ºC |
GTX225857-01 (50μl) |
GTX221667-01 EasyBlot anti-Mouse IgG (HRP) |
50μl |
-20 ºC |
GTX425858 EasyBlocker |
12g |
4 ºC |
|
Buffer
0.05M Tris (pH7.4), 0.15M NaCl, 1%BSA, 40% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.03 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Target Isotype
IgG
Purification
Purified by antigen-affinity chromatography.
Conjugation
Horseradish peroxidase(HRP)
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.