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EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G)

EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G). GTX225857-01

Cat. No. GTX225857-01

Reference 4

Application

 WB

Reactivity

 Mouse
Package
250 μl ($399),
50 μl ($169)
See all Mouse IgG products
PRODUCT

Summary

EasyBlot anti-Mouse IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of mouse IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP).

The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex’s EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.

Content

EasyBlot Kit Package Content Package Storage Temp
GTX225857-01 (250μl) GTX221667-01 EasyBlot anti-Mouse IgG (HRP) 250μl -20 ºC
GTX425858 EasyBlocker 60g 4 ºC
GTX225857-01 (50μl) GTX221667-01 EasyBlot anti-Mouse IgG (HRP) 50μl -20 ºC
GTX425858 EasyBlocker 12g 4 ºC

 

APPLICATION

Application Note

Suggested starting dilutions for WB is 1:1000. Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

PROPERTIES

Buffer

0.05M Tris (pH7.4), 0.15M NaCl, 1%BSA, 40% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G). GTX225857-01

GTX225857-01 WB Image

EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G) (GTX225857-01) was used as a secondary reagent for immunoprecipitation analysis.
HepG2 lysates were immunoprecipitated using 5 ug of normal rabbit IgG or ELP3 antibody [GT11811] (GTX631395).

REFERENCE

Application Reference

Takeuchi Y et al. Medicines (Basel) 2020; 7 (5) Rare Neurologic Disease-Associated Mutations of AIMP1 are Related with Inhibitory Neuronal Differentiation Which is Reversed by Ibuprofen.
Jean-Michel Paumier et al. FASEB 2018; (Epub) Proamyloidogenic effects of membrane type 1 matrix metalloproteinase involve MMP-2 and BACE-1 activities, and the modulation of APP trafficking.
Application : WB
Kubo Y et al. Front Microbiol 2017; 8 : 1653 Rab3a-Bound CD63 Is Degraded and Rab3a-Free CD63 Is Incorporated into HIV-1 Particles.
Application : WB
Lin TF et al. J Biol Chem 2014; The subfamily-specific assembly of Eag and Erg K+ channels is determined by both the amino and the carboxyl recognition domains.
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production