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EasyBlot anti Rabbit IgG (HRP)

EasyBlot anti Rabbit IgG (HRP). GTX221666-01
EasyBlot anti Rabbit IgG (HRP). GTX221666-01
EasyBlot anti Rabbit IgG (HRP). GTX221666-01
EasyBlot anti Rabbit IgG (HRP). GTX221666-01

Cat No. GTX221666-01

Application

WB

Reactivity

Rabbit
Package
250 μl ($199),
50 μl ($89)
PRODUCT

Summary

EasyBlot anti-Rabbit IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of mouse IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to rabbit IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Rabbit IgG (Optimized for Prot A/G) (GTXGTX225856-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Rabbit IgG Agarose beads (GTX27019) instead of Protein A/G.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000

Note :

WB
Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.

Not tested in other applications.

Positive Control

native-rabbit IgG

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA, 40% Glycerol. 0.005% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.318 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Target Isotype

IgG

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody (1) and EasyBlot anti-Rabbit IgG kit (GTX225856-01)(2). (A) Input, 20ug of HCT116 lysate. (B) and (C): HCT116 lysates were immunoprecipitated using 2.5 ug of normal rabbit IgG (B) or anti-p53 antibody (GTX102965)(C).  The precipitated protein was detected by GTX102965 diluted at 1:10000.

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody, EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) and EasyBlot anti-Rabbit IgG kit (GTX225856-01). A,C,and E: E. coli lysate with overexpressed GST-tagged protein B,D,and F: IP result IP and 1st antibody: anti-GST antibody

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody (GTX110736). The precipitated protein was detected by GTX110736 diluted at 1:3000.

A: EasyBlot
B: Goat anti-rabbit antibody

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

EasyBlot anti Rabbit IgG (HRP) antibody detects native-rabbit IgG by western blot analysis.Recombinant Protein (8 and 0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EasyBlot anti Rabbit IgG (HRP) antibody (GTX221666-01) at a dilution of 1:5000.

REFERENCE

REVIEW

EasyBlot anti Rabbit IgG (HRP) Cat No. GTX221666-01

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-Feb-2018
Score :
Application Tested : WB
Sample : HSC2 cells
Lane Description : Immunoprecipitation of Tel2 protein. Input, HSC2 cells (Lane 1). HSC2 cells subjected to immunoprecipitation using Tel2 antibody (Lane 2) or normal rabbit IgG (Lane 3). The precipitated protein was detected by RUVBL1 antibody. EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent in Western blot and can avoid the interference of IgG heavy chains and light chains.
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 199
$ 89