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EasyBlot anti Rabbit IgG (HRP)

EasyBlot anti Rabbit IgG (HRP). GTX221666-01
EasyBlot anti Rabbit IgG (HRP). GTX221666-01
EasyBlot anti Rabbit IgG (HRP). GTX221666-01
EasyBlot anti Rabbit IgG (HRP). GTX221666-01

Cat. No. GTX221666-01

Reference 49
1

Host

 Mouse

Clonality

 Monoclonal

Application

 WB

Reactivity

 Rabbit
Package
250 μl ($199),
50 μl ($89)
See all Rabbit IgG products
PRODUCT

Summary

EasyBlot anti-Rabbit IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of rabbit IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to rabbit IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Rabbit IgG (Optimized for Prot A/G) (GTXGTX225856-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Rabbit IgG Agarose beads (GTX27019) instead of Protein A/G.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000

Note :

WB
Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.
Not tested in other applications.

Positive Control

native-rabbit IgG

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Target Isotype

IgG

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_10620421

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody (1) and EasyBlot anti-Rabbit IgG kit (GTX225856-01)(2). (A) Input, 20ug of HCT116 lysate. (B) and (C): HCT116 lysates were immunoprecipitated using 2.5 ug of normal rabbit IgG (B) or anti-p53 antibody (GTX102965)(C).  The precipitated protein was detected by GTX102965 diluted at 1:10000.

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody, EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) and EasyBlot anti-Rabbit IgG kit (GTX225856-01). A,C,and E: E. coli lysate with overexpressed GST-tagged protein B,D,and F: IP result IP and 1st antibody: anti-GST antibody

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

EasyBlot anti Rabbit IgG (HRP) antibody detects native-rabbit IgG by western blot analysis.Recombinant Protein (8 and 0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EasyBlot anti Rabbit IgG (HRP) antibody (GTX221666-01) at a dilution of 1:5000.

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody (GTX110736). The precipitated protein was detected by GTX110736 diluted at 1:3000.

A: EasyBlot
B: Goat anti-rabbit antibody

REFERENCE

Application Reference

Zelinger L et al. iScience 2023; 26 (4) : 106417 Ultra-rare complement factor 8 coding variants in families with age-related macular degeneration.
June Seok Heo et al. Sci Rep 2022; 12 (1) : 2776 Human adipose mesenchymal stem cells modulate inflammation and angiogenesis through exosomes.
Application : WB
Stefanie Moritz et al. Cancers (Basel) 2021; 13 (21) Lamellipodin-RICTOR Signaling Mediates Glioblastoma Cell Invasion and Radiosensitivity Downstream of EGFR.
Application : WB
Laubscher D et al. Nat Commun 2021; 12 (1) : 6924 BAF complexes drive proliferation and block myogenic differentiation in fusion-positive rhabdomyosarcoma.
Application : WB
Zhou Y et al. BMC Vet Res 2021; 17 (1) : 289 Brucella melitensis UGPase inhibits the activation of NF-κB by modulating the ubiquitination of NEMO.
Application : WB
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REVIEW

EasyBlot anti Rabbit IgG (HRP) Cat. No. GTX221666-01

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
EasyBlot anti Rabbit IgG (HRP). GTX221666-01
Date : Anonymous submitted on 02-Feb-2018
Score :
Application Tested : WB
Sample : HSC2 cells
Lane Description : Immunoprecipitation of Tel2 protein. Input, HSC2 cells (Lane 1). HSC2 cells subjected to immunoprecipitation using Tel2 antibody (Lane 2) or normal rabbit IgG (Lane 3). The precipitated protein was detected by RUVBL1 antibody. EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent in Western blot and can avoid the interference of IgG heavy chains and light chains.
Submit a Review
Application Reference
Zelinger L et al. iScience 2023; 26 (4):106417 Ultra-rare complement factor 8 coding variants in families with age-related macular degeneration.
June Seok Heo et al. Sci Rep 2022; 12 (1):2776 Human adipose mesenchymal stem cells modulate inflammation and angiogenesis through exosomes.
Application : WB
Stefanie Moritz et al. Cancers (Basel) 2021; 13 (21) Lamellipodin-RICTOR Signaling Mediates Glioblastoma Cell Invasion and Radiosensitivity Downstream of EGFR.
Application : WB
Laubscher D et al. Nat Commun 2021; 12 (1):6924 BAF complexes drive proliferation and block myogenic differentiation in fusion-positive rhabdomyosarcoma.
Application : WB
Zhou Y et al. BMC Vet Res 2021; 17 (1):289 Brucella melitensis UGPase inhibits the activation of NF-κB by modulating the ubiquitination of NEMO.
Application : WB
Niu B et al. Nat Commun 2021; 12 (1):2809 Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis.
Application : WB
Luanpitpong S et al. Stem Cells 2021; Metabolic sensor O-GlcNAcylation regulates megakaryopoiesis and thrombopoiesis through c-Myc stabilization and integrin perturbation.
Application : WB
Lo H.F. et al. bioRxiv 2021; (Epub) Δ9-tetrahydrocannabinol inhibits Hedgehog-dependent patterning during development.
Application : WB
Deville SS et al. Int J Mol Sci 2020; 21 (19) c-Abl Tyrosine Kinase Is Regulated Downstream of the Cytoskeletal Protein Synemin in Head and Neck Squamous Cell Carcinoma Radioresistance and DNA Repair.
Gao S et al. Nat Genet 2020; 52 (10):1011-1017 Chromatin binding of FOXA1 is promoted by LSD1-mediated demethylation in prostate cancer.
Application : WB
Tao F et al. Ann Neurol 2019; 85 (3):316-330 Variation in SIPA1L2 is correlated with phenotype modification in Charcot- Marie- Tooth disease type 1A.
Li J et al. Theranostics 2019; 9 (1):179-195 OTUB2 stabilizes U2AF2 to promote the Warburg effect and tumorigenesis via the AKT/mTOR signaling pathway in non-small cell lung cancer.
Lin MW et al. Neurotherapeutics 2019; (Epub) Galantamine Inhibits Aβ1-42-Induced Neurotoxicity by Enhancing α7nAChR Expression as a Cargo Carrier for LC3 Binding and Aβ1-42 Engulfment During Autophagic Degradation.
Application : WB
Mukohda M et al. J Clin Invest 2019; 129 (6):2318-2332 RhoBTB1 protects against hypertension and arterial stiffness by restraining phosphodiesterase 5 activity.
Mathieu Letellier et al. bioRxiv 2019; (Epub) Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation.
Application : WB
Huang X et al. Cancer Immunol Res 2019; (Epub) USP22 deubiquitinates CD274 to suppress anti-cancer immunity.
Kamijo A et al. Histochem Cell Biol 2019; (Epub) Scaffold protein Lin7 family in membrane skeletal protein complex in mouse seminiferous tubules.
Application : WB
Shiau MY et al. Sci Rep 2019; 9 (1):11974 Mechanism of Interleukin-4 Reducing Lipid Deposit by Regulating Hormone-Sensitive Lipase.
Application : WB
McComb S et al. Sci Adv 2019; 5 (7):eaau9433 Efficient apoptosis requires feedback amplification of upstream apoptotic signals by effector caspase-3 or -7.
Application : WB
Nayak A et al. Cell Rep 2019; 27 (9):2725-2736.e4 Regulation of SETD7 Methyltransferase by SENP3 Is Crucial for Sarcomere Organization and Cachexia.
Application : WB
Cheng WC et al. Int J Cancer 2019; (Epub) RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer.
Application : WB
Ringeard M et al. Nature 2019; 565 (7740):500-504 FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.
Application : WB
Huang X et al. Autophagy 2019;:1-22 The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.
Application : WB
Richards T et al. Biochim Biophys Acta Mol Basis Dis 2019; 1865 (2):378-390 Atmin modulates Pkhd1 expression and may mediate Autosomal Recessive Polycystic Kidney Disease (ARPKD) through altered non-canonical Wnt/Planar Cell Polarity (PCP) signalling.
Application : WB
Letellier M et al. Nat Commun 2018; 9 (1):3979 A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation.
Altamura G et al. Sci Rep 2018; 8 (1):17529 Felis catus papillomavirus type-2 E6 binds to E6AP, promotes E6AP/p53 binding and enhances p53 proteasomal degradation.
Application : WB
Su Z et al. Mol Cancer Ther 2018; (Epub) Anti-MET VHH Pool Overcomes MET-Targeted Cancer Therapeutic Resistance.
Application : WB
Chen YL et al. Nature 2018; 564 (7734):125-129 TIC236 links the outer and inner membrane translocons of the chloroplast.
Application : WB
Mathieu Letellier et al. Nature Communication 2018; 9:3979 A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation.
Application : WB
Huang SC et al. EMBO Mol Med 2017; (Epub) TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.
Application : WB
Gdula-Argasi?ska J et al. Acta Biochim Pol 2017; 64 (3):551-559 Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells.
Application : WB
ZAJAC A et al. Medicina Internacia Revuo 2017; 28 (108):176-181 Influence of αlpha-linolenic acid supplementation on murine macrophages RA W 264.7 activated with lipopolisaccharide.
Application : WB
Lodato P.B. et al. FEMS Microbiol Lett 2017; (Epub) Effect of RNase E deficiency on translocon protein synthesis in an RNase E-inducible strain of enterohemorrhagic Escherichia coli O157:H7.
Application : WB
Torii S et al. EMBO Rep 2016; (Epub) Identification of PPM1D as an essential Ulk1 phosphatase for genotoxic stress-induced autophagy.
Application : WB
Gdula-Argasi?ska J et al. Toxicol Lett 2016; 258:93-100 Docosahexaenoic acid attenuates in endocannabinoid synthesis in RAW 264.7 macrophages activated with benzo(a)pyrene and lipopolysaccharide.
Application : WB
Gdula-Argasi?ska J et al. Pharmacol Rep 2016; 68 (5):939-944 Resolvin D1 down-regulates CYP1A1 and PTGS2 gene in the HUVEC cells treated with benzo(a)pyrene.
Application : WB
Barel M et al. Cell Microbiol 2016; (Epub) Host glycosylation pathways and the unfolded protein response contribute to the infection by Francisella.
Application : WB
Muszy?ska B et al. Pharm Biol 2016;:43111 In vitro cultures of Bacopa monnieri and an analysis of selected groups of biologically active metabolites in their biomass.
Application : WB
Xing C et al. Cancer Res 2016; 76 (1):83-95 Ubiquitin-Specific Protease 4-Mediated Deubiquitination and Stabilization of PRL-3 Is Required for Potentiating Colorectal Oncogenesis.
Wang B et al. Nat Commun 2015; 6:8704 POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.
Application : WB
Kolodney G et al. J Biol Chem 2015; 290 (52):31037-50 Nuclear Compartmentalization of Serine Racemase Regulates d-Serine Production: IMPLICATIONS FOR N-METHYL-d-ASPARTATE (NMDA) RECEPTOR ACTIVATION.
Joanna Gdula-Argasin’ ska et al. Pharmacol Rep. 2015; 67 (3):610-5 n-3 fatty acids as resolvents of inflammation in the A549 cells.
Application : WB
Shang ZF et al. Int J Biol Sci 2015; 11 (9):1026-35 DNA-PKcs Negatively Regulates Cyclin B1 Protein Stability through Facilitating Its Ubiquitination Mediated by Cdh1-APC/C Pathway.
Application : WB
Chang Y et al. J Agric Food Chem. 2015; Monascus Secondary Metabolites Monascin and Ankaflavin Inhibit Activation of RBL-2H3 Cells.
Application : WB
Kanzaki H et al. Free Radic Biol Med 2014; Nuclear Nrf2 Induction by Protein Transduction Attenuates Osteoclastogenesis.
Application : WB
Jiang S et al. Mol Cell Biol 2014; Piwil2 inhibits Keratin 8 degradation through promoting p38-induced phosphorylation to resist Fas-mediated apoptosis.
Application : WB
Wu MH et al. Oncogene 2014; (Epub) MCT-1 expression and PTEN deficiency synergistically promote neoplastic multinucleation through the Src/p190B signaling activation.
Application : WB
Huang TK et al. Plant Cell 2013; 25 (10):4044-60 Identification of downstream components of ubiquitin-conjugating enzyme PHOSPHATE2 by quantitative membrane proteomics in Arabidopsis roots.
Application : WB
Huang JT et al. J Virol 2013; 87 (9):4994-5004 Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase.
Application : WB
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 199
$ 89