Content
| EasyBlot Kit Package |
Content |
Package |
Storage Temp |
| GTX228911-01 (250μl) |
GTX628906-01 EasyBlot anti-Sheep IgG (HRP) |
250μl |
-20 ºC |
| GTX425858 EasyBlocker |
62g |
4 ºC |
| GTX228911-01 (50μl) |
GTX628906-01 EasyBlot anti-Sheep IgG (HRP) |
50μl |
-20 ºC |
| GTX425858 EasyBlocker |
12g |
4 ºC |
|
Summary
EasyBlot anti-Sheep IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP).
The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex’s EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.
Note: Suggested dilution is 1:1000. Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples.
Application Note
Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Positive Control
0.2 μg native sheep IgG , 0.1 μg native sheep IgG
Form
Liquid
Buffer
0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA, 40% Glycerol. 0.005% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.18 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Sheep
Target Isotype
IgG
Purification
Affinity purified by Protein G.
Conjugation
Horseradish peroxidase(HRP)
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
