EasyBlot anti-Sheep IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of Sheep IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to sheep IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.
To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-sheep IgG (Optimized for Prot A/G) (GTX228911-01) or EasyBloker (GTX425858) to minimize protein A/G contamination.
*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
0.2 μg native sheep IgG , 0.1 μg native sheep IgG
0.05M Tris (pH7.4), 0.15M NaCl, 1%BSA, 40% Glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.18 mg/ml (Please refer to the vial label for the specific concentration.)
Affinity purified by Protein G.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.