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Ebola virus GP1 antibody

Anti-Ebola virus GP1 antibody used in Western Blot (WB). GTX134144
Anti-Ebola virus GP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134144

Cat No. GTX134144

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Ebola virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

Zaire ebolavirus GP-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Ebola virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Ebola virus GP1 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Ebola virus GP1 antibody used in Western Blot (WB). GTX134144

GTX134144 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ebola virus GP1 antibody (GTX134144) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ebola virus GP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134144

GTX134144 ICC/IF Image

Ebola virus GP1 antibody detects Ebola virus GP1 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ebola virus GP1 protein stained by Ebola virus GP1 antibody (GTX134144) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf