GeneTex
United States (US)

Ebola virus GP2-delta antibody

GTX134145_42977_20171117_IFA_18121410_140.jpg
GTX134145_42977_20170915_WB_B_18121410_479.jpg

Cat No. GTX134145

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Ebola virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

Zaire ebolavirus GP-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Ebola virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Ebola virus GP2-delta protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX134145_42977_20171117_IFA_18121410_140.jpg

GTX134145 ICC/IF Image

Ebola virus GP2-delta antibody detects Ebola virus GP2-delta protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ebola virus GP2-delta protein stained by Ebola virus GP2-delta antibody (GTX134145) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX134145_42977_20170915_WB_B_18121410_479.jpg

GTX134145 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ebola virus GP2-delta antibody (GTX134145) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169