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Ebola virus GP2-delta antibody

Anti-Ebola virus GP2-delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134145
Anti-Ebola virus GP2-delta antibody used in Western Blot (WB). GTX134145

Cat No. GTX134145

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Ebola virus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

Zaire ebolavirus GP-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Ebola virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Ebola virus GP2-delta protein (Zaire ebolavirus (strain Kikwit-95)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887234

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ebola virus GP protein , EBOV GP protein

Research Area

DATA IMAGES

Anti-Ebola virus GP2-delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134145

GTX134145 ICC/IF Image

Ebola virus GP2-delta antibody detects Ebola virus GP2-delta protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ebola virus GP2-delta protein stained by Ebola virus GP2-delta antibody (GTX134145) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Ebola virus GP2-delta antibody used in Western Blot (WB). GTX134145

GTX134145 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ebola virus GP2-delta antibody (GTX134145) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf