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Ebola virus NP antibody

Anti-Ebola virus NP antibody used in Western Blot (WB). GTX134031

Cat. No. GTX134031

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Ebola virus
Package
100 μl ($399),
25 μl ($169)
See all Ebola virus NP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

DDDDK-tagged Zaire ebolavirus NP-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Ebola virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Ebola virus NP protein (Zaire ebolavirus (strain Mayinga-76)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887180

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ebola virus Nucleoprotein , Ebola virus NP , EBOV Nucleoprotein , EBOV NP

Research Area

DATA IMAGES

Anti-Ebola virus NP antibody used in Western Blot (WB). GTX134031

GTX134031 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ebola virus NP antibody (GTX134031) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Vidal S et al. mBio 2021; 12 (4) : e0097221 Expression of the Ebola Virus VP24 Protein Compromises the Integrity of the Nuclear Envelope and Induces a Laminopathy-Like Cellular Phenotype.
Application : WB
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REVIEW

Ebola virus NP antibody Cat. No. GTX134031

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Ebola virus NP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134031
Date : Reika Watanabe submitted on 13-Jan-2022
Score :
Application Tested : ICC/IF
Sample : HEK293T expressing Ebola Zaire NP
Fixation : -20 degree Methanol
Permeabilization /Antigen retrieval : no additional permeabilization
Primary Antibody : 1:300, room temperature°C, 1Hr
Figure Note : HEK293T cells transiently expressing Ebola Zaire NP and control non-transfected HEK293T cells were fixed by cold methanol (-20 degree). The cells were stained with primary anti-NP antibody (1:300) at room temperature for 1 hr followed by secondary antibody (anti-Rabbit conjugated with Alexa 488). Images are observed by wide-filed fluorescence microscopy using a 40 x objective lens.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169