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Ebola virus VP35 antibody

Anti-Ebola virus VP35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134032
Anti-Ebola virus VP35 antibody used in Western Blot (WB). GTX134032

Cat No. GTX134032

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Ebola virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

Zaire ebolavirus VP35-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Ebola virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Ebola virus VP35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ebola VP35 antibody

Research Area

DATA IMAGES
Anti-Ebola virus VP35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134032

GTX134032 ICC/IF Image

Ebola virus VP35 antibody detects Ebola virus VP35 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ebola virus VP35 protein stained by Ebola virus VP35 antibody (GTX134032) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Ebola virus VP35 antibody used in Western Blot (WB). GTX134032

GTX134032 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with Ebola virus VP35 antibody (GTX134032) diluted at 1:5000 and DDDDK tag antibody (GTX115043) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf