GeneTex
United States (US)

Ef1a antibody

GTX124538_39995_WB_Z_18121410_986.jpg
GTX124538_39995_IHC_Z_18121410_857.jpg

Cat No. GTX124538

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

zebrafish embryo
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Elongation Factor 1 Alpha 1, Like 1,Efl1-Alpha,Chunp6927,Eef1A,Ef1A,Ik:Tdsubc_2A3,Ik:Tdsubc_2B3,Tdsubc_2A3,Wu:Fa91C07,Wu:Fa94B03,Wu:Fi13B09,Xx:Tdsubc_2A3,Xx:Tdsubc_2B3,Eef1A1L1

Cellular Localization

Cytoplasm

Background

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Database

Research Area

DATA IMAGES
GTX124538_39995_WB_Z_18121410_986.jpg

GTX124538 WB Image

Sample (30 µg of whole cell lysate)
A: zebrafish embryo
10% SDS PAGE
GTX124538 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124538_39995_IHC_Z_18121410_857.jpg

GTX124538 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ef1a (GTX124538) antibody at 1:300 dilution.

REFERENCE
REVIEW

Ef1a antibody Cat No. GTX124538

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 14-Aug-2013
Score :
Application Tested : WB
Sample Species : Zfsh
Sample : Liver lysate of adult zebrafish
Amount used : 5μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 389
$ 199