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Eg5 antibody [C2C3], C-term

Anti-Eg5 antibody [C2C3], C-term used in Western Blot (WB). GTX109054
Anti-Eg5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109054
Anti-Eg5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109054
Anti-Eg5 antibody [C2C3], C-term used in Western Blot (WB). GTX109054

Cat. No. GTX109054

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

H1299 , HeLaS3

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Eg5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950620

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinesin family member 11 , EG5 , HKSP , KNSL1 , MCLMR , TRIP5

Background

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Eg5 antibody [C2C3], C-term used in Western Blot (WB). GTX109054

GTX109054 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: HeLaS3
7.5% SDS PAGE
GTX109054 diluted at 1:5000

Anti-Eg5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109054

GTX109054 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Eg5 (GTX109054) antibody at 1:200 dilution.

Anti-Eg5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109054

GTX109054 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Eg5(GTX109054) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Eg5 antibody [C2C3], C-term used in Western Blot (WB). GTX109054

GTX109054 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27447563
The data was published in the journal Oncotarget in 2016. PMID: 27447563

REFERENCE

REVIEW

Eg5 antibody [C2C3], C-term Cat. No. GTX109054

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27447563
Date : Anonymous submitted on 17-Jul-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human mammary epithelial cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169