GeneTex

  • Country / Location Selection

United States (US)

Eg5 antibody

Anti-Eg5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30692
Anti-Eg5 antibody used in Immunohistochemistry (IHC). GTX30692

Cat. No. GTX30692

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat, Drosophila, Pig, Primate
Package
100 μl ($439)
See all Eg5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:1000
IP 1:10 - 1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A recombinant segment of the coiled-coil domain of human Eg5 [UniProt# P52732]

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_625879

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinesin family member 11 , EG5 , HKSP , KNSL1 , MCLMR , TRIP5

Cellular Localization

Cytoplasm

Background

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Eg5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30692

GTX30692 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30692 Eg5 antibody. Cytoplasmic staining during interphase and spindle staining during mitosis.
Fixation : 3.5% PFA

Anti-Eg5 antibody used in Immunohistochemistry (IHC). GTX30692

GTX30692 IHC Image

The data was published in the journal Nat Commun in 2018. PMID: 29899413

REFERENCE

Application Reference

Freixo F et al. Nat Commun 2018; 9 (1) : 2330 NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization.
Eibes S et al. Curr Biol 2018; 28 (1) : 121-129.e4 Nek9 Phosphorylation Defines a New Role for TPX2 in Eg5-Dependent Centrosome Separation before Nuclear Envelope Breakdown.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Eg5 antibody (GTX30692). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 439