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Eif2s1 antibody

Anti-Eif2s1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124488
Anti-Eif2s1 antibody used in Western Blot (WB). GTX124488

Cat. No. GTX124488

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Eif2s1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

whole zebrafish , zebrafish eye

Predict Reactivity

Human (100%)| Mouse (98%)| Rat (98%)| Bovine (98%)| Chicken (97%)| Xenopus tropicalis (93%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Eif2s1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167127

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2, subunit 1 alpha b , eif2 , eif2s1 , fb19d01 , wu:fb19d01

Database

Research Area

DATA IMAGES

Anti-Eif2s1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124488

GTX124488 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish kidney tube, using eif2s1 (GTX124488) antibody at 1:300 dilution.

Anti-Eif2s1 antibody used in Western Blot (WB). GTX124488

GTX124488 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Eif2s1 antibody (GTX124488) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lee HC et al. Int J Mol Sci 2023; 24 (24) The Upstream 1350~1250 Nucleotide Sequences of the Human ENDOU-1 Gene Contain Critical Cis-Elements Responsible for Upregulating Its Transcription during ER Stress.
Zhao S et al. Chemosphere 2018; (Epub) N-acetylcysteine protects against microcystin-LR-induced endoplasmic reticulum stress and germ cell apoptosis in zebrafish testes.
Application : WB
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169