Immunohistochemical analysis of paraffin-embedded zebrafish kidney tube, using eif2s1 (GTX124488) antibody at 1:300 dilution.

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Eif2s1 antibody (GTX124488) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.