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Elastase 3A antibody

Anti-Elastase 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX104918
Anti-Elastase 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX104918
Anti-Elastase 3A antibody used in Western Blot (WB). GTX104918

Cat. No. GTX104918

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Elastase 3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

PC-3

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Elastase 3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163724

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chymotrypsin like elastase family member 3A , ELA3 , ELA3A

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Elastase 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX104918

GTX104918 IHC-P Image

ELA3A antibody detects ELA3A protein at cytoplasm in human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma.
ELA3A antibody (GTX104918) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Elastase 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX104918

GTX104918 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ELA3A(GTX104918) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Elastase 3A antibody used in Western Blot (WB). GTX104918

GTX104918 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
12% SDS PAGE
GTX104918 diluted at 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169