PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Pancreas: Acinar cells should show a strong CELA3B immunostaining with apical predominance.
Negative Control
Kidney: CELA3B staining should be absent in all epithelial cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human CELA3B protein fragment (around aa 82-238)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chymotrypsin like elastase family member 3B , CBPP , ELA3B
Background
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009]
Database
Research Area
DATA IMAGES
![Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445](/templates/default/js/lightbox/images/loading.gif "Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445")
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GTX04445 IHC-P Image
IHC-P analysis of human acinar cell carcinoma (ACC) from pancreas tissue using GTX04445 Elastase 3B antibody [MSVA-410M] HistoMAX™.
Acinar cell carcinoma of the pancreas exhibiting a strong elastase 3B immunostaining of all tumor cells.
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GTX04445 IHC-P Image
IHC-P analysis of human kidney tissue using GTX04445 Elastase 3B antibody [MSVA-410M] HistoMAX™.
Elastase 3B immunostaining is absent in kidney tissue.
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GTX04445 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX04445 Elastase 3B antibody [MSVA-410M] HistoMAX™.
Normal pancreas with strong elastase 3B positivity of acinar cells.
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REFERENCE
There are currently no references for Elastase 3B antibody [MSVA-410M] HistoMAX™ (GTX04445). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Elastase 3B antibody [MSVA-410M] HistoMAX™ (GTX04445). Be the first to share your experience with this product.
![Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445](/upload/website/prouct_img/small/GTX04445/GTX04445_20230728_IHC-P_156_23072722_456.webp "Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445")
![Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445](/upload/website/prouct_img/small/GTX04445/GTX04445_20230728_IHC-P_286_23072723_614.webp "Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445")
![Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445](/upload/website/prouct_img/small/GTX04445/GTX04445_20230728_IHC-P_26_23072722_767.webp "Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445")
![Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445](/upload/website/prouct_img/normal/GTX04445/GTX04445_20230728_IHC-P_156_23072722_456.webp "Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445")
![Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445](/upload/website/prouct_img/normal/GTX04445/GTX04445_20230728_IHC-P_286_23072723_614.webp "Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445")
![Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445](/upload/website/prouct_img/normal/GTX04445/GTX04445_20230728_IHC-P_26_23072722_767.webp "Anti-Elastase 3B antibody [MSVA-410M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04445")