Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Elastase 3B antibody (GTX115702) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (22.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Elastase 3B antibody (GTX115702) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.