Elavl3+4 antibody

Name: Elavl3+4 antibody
Brand: GeneTex
SKU: GTX128365
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

Cat. No. GTX128365

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	ICC/IF, IHC-Fr, IHC-Wm
Reactivity	Human, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all zebrafish Elavl3+4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	Assay dependent
IHC-Fr	Assay dependent
IHC-Wm	1:100-1:500

Not tested in other applications.

Predict Reactivity

Mouse, Rat, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Elavl3+4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885764

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Elavl3 antibody , HuD antibody , HuC/D antibody , HuC antibody , wu:fb77b03 antibody , id:ibd1248 antibody , elrc antibody , zHuC antibody , ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) antibody , ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) antibody

Background

Implicated in tumor angiogenesis (By similarity). May inhibits BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. May play a role during myoblast and osteoblast differentiation, and maturation.

Research Area

- Zebrafish

DATA IMAGES

GTX128365 IHC-Wm Image

Elavl3+4 antibody detects Elavl3+4 protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Elavl3+4 antibody (GTX128365) dilution: 1:200.

GTX128365 IHC-Wm Image

Elavl3+4 antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Elavl3+4 antibody (GTX128365) dilution: 1:100.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-2] (GTX16292) dilution: 1:25.

GTX128365 IHC-Wm Image

Elavl3+4 antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
Green: Elavl3+4 antibody (GTX128365) dilution: 1:100.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-2] (GTX16292) dilution: 1:25.

GTX128365 IHC-Wm Image

Elavl3+4 antibody detects Elavl3+4 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Elavl3+4 stained by Elavl3+4 antibody (GTX128365) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

Application Reference

Ilaria Dutto et al. Elife 2022; 11 Pathway-specific effects of ADSL deficiency on neurodevelopment.

Application : ICC/IF

Reactivity : Human

Vera L Hopfenm?ller et al. Front Cell Dev Biol 2021; 9 : 809962 The Wilms Tumor Gene wt1a Contributes to Blood-Cerebrospinal Fluid Barrier Function in Zebrafish.

Application : IHC-Fr

Reactivity : Zebrafish

Li X et al. Stem Cell Res Ther 2019; 10 (1) : 389 SOX19b regulates the premature neuronal differentiation of neural stem cells through EZH2-mediated histone methylation in neural tube development of zebrafish.

Application : IHC-Fr

Reactivity : Zebrafish

Berg EM et al. Brain Struct Funct 2018; (Epub) Complementary expression of calcium binding proteins delineates the functional organization of the locomotor network.

Application : IHC-Wm

Reactivity : Zebrafish

Amamoto R et al. Elife 2016; 5 Adult axolotls can regenerate original neuronal diversity in response to brain injury.

Application : IHC-Fr

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