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Elavl3+4 antibody

Anti-Elavl3+4 antibody used in Western Blot (WB). GTX128365
Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365
Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365
Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365
Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365
Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

Cat No. GTX128365

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr, IHC-Wm

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

zebrafish brain , zebrafish eye , whole zebrafish

Predict Reactivity

Human, Mouse, Rat, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.67 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Elavl3+4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885764

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Elavl3 antibody , HuD antibody , HuC/D antibody , HuC antibody , wu:fb77b03 antibody , id:ibd1248 antibody , elrc antibody , zHuC antibody , ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) antibody , ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) antibody

Background

Implicated in tumor angiogenesis (By similarity). May inhibits BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. May play a role during myoblast and osteoblast differentiation, and maturation.

Research Area

DATA IMAGES

Anti-Elavl3+4 antibody used in Western Blot (WB). GTX128365

GTX128365 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Elavl3+4 antibody (GTX128365) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody detects Elavl3+4 protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Elavl3+4 antibody (GTX128365) dilution: 1:200.

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Elavl3+4 antibody (GTX128365) dilution: 1:100.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-2] (GTX16292) dilution: 1:25.

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
Green: Elavl3+4 antibody (GTX128365) dilution: 1:100.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-2] (GTX16292) dilution: 1:25.

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Elavl3+4 antibody (GTX128365) dilution: 1:100.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-2] (GTX16292) dilution: 1:25.

Anti-Elavl3+4 antibody used in IHC (Whole mount) (IHC-Wm). GTX128365

GTX128365 IHC-Wm Image

Elavl3+4 antibody detects Elavl3+4 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Elavl3+4 stained by Elavl3+4 antibody (GTX128365) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

REVIEW

Elavl3+4 antibody Cat No. GTX128365

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 17-Jul-2019
Score :
Application Tested : IHC-Fr
Sample : Adult mouse brain tissue
Blocking : 1% BSA in PBS, 25°C, 0.5Hr
Fixation : 4% praformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.3% Triton X -100 in PBS
Primary Antibody : 1:200, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 409
$ 219
Currently in production