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Elongin B antibody

Anti-Elongin B antibody used in Immunoprecipitation (IP). GTX16427
Anti-Elongin B antibody used in Western Blot (WB). GTX16427

Cat No. GTX16427

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human TCEB2 (NP_009039.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Elongin B,Siii,Tceb2,Elob

Background

This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq, Aug 2008]

Database

DATA IMAGES
Anti-Elongin B antibody used in Immunoprecipitation (IP). GTX16427

GTX16427 IP Image

IP analysis of MCF7 cell lysate using GTX16427 Elongin B antibody.
IP reaction : 3μg antibody / 150μg cell lysate
Dilution : 1:500

Anti-Elongin B antibody used in Western Blot (WB). GTX16427

GTX16427 WB Image

WB analysis of various samples using GTX16427 Elongin B antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 319