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Emerin antibody [4G5]

Anti-Emerin antibody [4G5] used in IHC (Paraffin sections) (IHC-P). GTX01975

Cat. No. GTX01975

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G5

Isotype

 IgG1

Application

 ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
500 μl ($659)
See all Emerin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:160
IHC-Fr 1:20–1:40

Note :

IHC-P
The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections. Heat Induced Epitope Retrieval (HIER) was performed in Epitope Retrieval Solution pH 6.

IHC-Fr
This antibody requires the sections to be fixed in acetone for 10 minutes at 25ºC prior to incubation with the primary antibody.
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Prokaryotic recombinant protein corresponding to a 222 amino acid region near the N-terminus of the emerin protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

emerin , EDMD , LEMD5 , STA

Cellular Localization

Nucleus inner membrane , Nucleus outer membrane

Background

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Emerin antibody [4G5] used in IHC (Paraffin sections) (IHC-P). GTX01975

GTX01975 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX01975 Emerin antibody [4G5]. Note perinuclear staining of all cell nuclei.

REFERENCE

Clone Reference

Ji-Yeon Shin et al. 2013; 26 (6) Lamina-associated polypeptide-1 interacts with the muscular dystrophy protein emerin and is essential for skeletal muscle maintenance
Application : ICC/IF
Reactivity : Mouse
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REVIEW

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Package List Price ($)
$ 659