Application Note
WB: Use at a concentration of 1 - 2 μg/ml. IHC-P: Use at a concentration of 10-15μg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa), determined using human HepG2 (hepatoblastoma) cell lysate. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Positive Control
HepG2 cell lysate
Specificity/Sensitivity
This antibody recognizes human, mouse, and rat EndoG (Endonuclease G).
Form
Liquid
Buffer
Preservative: 0.02% Sodium Azide; Constituents: PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide: GGPRGPGELAKYGLP, corresponding to amino acids 55-70 of Human Endonuclease G.
Purification
Immunogen affinity purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
Endonuclease G,Endog
Cellular Localization
Mitochondrial
Background
The activities of caspase and nuclease are involved in DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Studies have demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
Database
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