GeneTex
United States (US)

EndoG antibody

Endonuclease G antibody_Western blot_GTX10522-1_19011720_147.jpg
Endonuclease G antibody_IHC (Formalin-fixed paraffin-embedded sections)_GTX10522-1_19011720_218.jpg

Cat No. GTX10522

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

WB: Use at a concentration of 1 - 2 μg/ml. IHC-P: Use at a concentration of 10-15μg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa), determined using human HepG2 (hepatoblastoma) cell lysate. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

33 kDa. ( Note )

Positive Control

HepG2 cell lysate

Specificity/Sensitivity

This antibody recognizes human, mouse, and rat EndoG (Endonuclease G).
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: GGPRGPGELAKYGLP, corresponding to amino acids 55-70 of Human Endonuclease G.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Endonuclease G,Endog

Cellular Localization

Mitochondrial

Background

The activities of caspase and nuclease are involved in DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Studies have demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.

Database

DATA IMAGES
Endonuclease G antibody_Western blot_GTX10522-1_19011720_147.jpg

GTX10522 WB Image

Western blot analysis of whole cell lysates from mouse 3T3 (M) and Human HepG2 (H) cells probed with Rabbit anti endonuclease G (GTX10522)

Endonuclease G antibody_IHC (Formalin-fixed paraffin-embedded sections)_GTX10522-1_19011720_218.jpg

GTX10522 IHC-P Image

Staining of formalin fixed, paraffin processed human pancreas with Rabbit anti Human EndoG at μg/ml. (GTX10522)

Package List Price ($)
$ 319