After AP reaction GTX30968 is added to chromogen system as follows:
1. Chromogen buffer and substrates for AP are available as ready-to-use (one component), two components or three components. Mix according to manufacture instructions.
2. To one ml of this ready to use buffer, chromogen and substrate mixture, add two drop of GTX30968, mix well for few seconds.
3. Add this chromogen mixture to your specimen incubate according to manufactures instructions.