GeneTex
United States (US)

Endomucin antibody [N1C2]

GTX101871_WB_19011506_179.jpg
GTX101871_39609_IHC_19011506_413.jpg

Cat No. GTX101871

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Endomucin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EMCN2 antibody, MUC14 antibody, EMCN antibody, endomucin-2 antibody, endomucin antibody, gastric cancer antigen Ga34 antibody, MUC-14 antibody, mucin-14 antibody

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX101871_WB_19011506_179.jpg

GTX101871 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX101871 diluted at 1:1000

GTX101871_39609_IHC_19011506_413.jpg

GTX101871 IHC-P Image

Endomucin antibody [N1C2] detects Endomucin protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Endomucin antibody [N1C2] (GTX101871) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf