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Endomucin antibody [N1C2]

Anti-Endomucin antibody [N1C2] used in Western Blot (WB). GTX101871
Anti-Endomucin antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101871

Cat No. GTX101871

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Endomucin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endomucin , EMCN2 , MUC14

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Endomucin antibody [N1C2] used in Western Blot (WB). GTX101871

GTX101871 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX101871 diluted at 1:1000

Anti-Endomucin antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101871

GTX101871 IHC-P Image

Endomucin antibody [N1C2] detects Endomucin protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Endomucin antibody [N1C2] (GTX101871) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for Endomucin antibody [N1C2] (GTX101871). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Endomucin antibody [N1C2] (GTX101871). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169