FACS analysis of HEK293T cells transfected with either Endothelial Lipase / LIPG plasmid(Red) or empty vector control plasmid(Blue) using GTX84209 Endothelial Lipase / LIPG antibody [1B8].

ICC/IF analysis of COS7 cells transiently transfected with Endothelial Lipase / LIPG plasmid using GTX84209 Endothelial Lipase / LIPG antibody [1B8].

IP analysis of DDDDK tagged Endothelial Lipase / LIPG overexpressed HEK293T lysate using GTX84209 Endothelial Lipase / LIPG antibody [1B8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

WB analysis of various cell lines using GTX84209 Endothelial Lipase / LIPG antibody [1B8].
Loading : 35 ug per lane
Dilution : 1:200

IHC-P analysis of human kidney tissue using GTX84209 Endothelial Lipase / LIPG antibody [1B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of HEK293T cells transfected with Endothelial Lipase / LIPG plasmid (Right) or empty vector (Left) for 48 hrs using GTX84209 Endothelial Lipase / LIPG antibody [1B8].
Loading : 5 ug per lane