Endothelial Lipase / LIPG antibody [4A8]

Name: Endothelial Lipase / LIPG antibody [4A8]
Brand: GeneTex
SKU: GTX84191
Price: 519.00 USD

Anti-Endothelial Lipase / LIPG antibody [4A8] used in Flow cytometry (FACS). GTX84191

Anti-Endothelial Lipase / LIPG antibody [4A8] used in IHC (Paraffin sections) (IHC-P). GTX84191

Anti-Endothelial Lipase / LIPG antibody [4A8] used in Western Blot (WB). GTX84191

Anti-Endothelial Lipase / LIPG antibody [4A8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84191

Anti-Endothelial Lipase / LIPG antibody [4A8] used in Immunoprecipitation (IP). GTX84191

Cat. No. GTX84191

Host	Mouse
Clonality	Monoclonal
Clone Name	4A8
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS, IP
Reactivity	Human, Mouse, Monkey
Package	100 μl ($519)

See all Endothelial Lipase / LIPG products

Datasheet
Document

View product citations for antibody GTX84191 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:50
FACS	1:100
IP	2ug/500ul

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human LIPG (NP_006024) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lipase G, endothelial type , EDL , EL , PRO719

Cellular Localization

Secreted

Background

Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.

Database

Gene ID: 9388 LIPG
UniProt: Q9Y5X9 LIPG

Research Area

DATA IMAGES

GTX84191 FACS Image

FACS analysis of Jurkat cells using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Red : Primary antibody
Blue : Negative control antibody

GTX84191 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84191 WB Image

WB analysis of various cell lines and human liver tissue lysate using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Loading : 10 ug per lane
Dilution : 1:200

GTX84191 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84191 FACS Image

FACS analysis of HeLa cells using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Red : Primary antibody
Blue : Negative control antibody

GTX84191 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84191 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84191 IHC-P Image

IHC-P analysis of human liver tissue using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84191 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84191 FACS Image

FACS analysis of HEK293T cells transfected with either Endothelial Lipase / LIPG plasmid(Red) or empty vector control plasmid(Blue) using GTX84191 Endothelial Lipase / LIPG antibody [4A8].

GTX84191 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Endothelial Lipase / LIPG plasmid using GTX84191 Endothelial Lipase / LIPG antibody [4A8].

GTX84191 IP Image

IP analysis of DDDDK tagged Endothelial Lipase / LIPG overexpressed HEK293T lysate using GTX84191 Endothelial Lipase / LIPG antibody [4A8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX84191 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84191 WB Image

WB analysis of HEK293T cells transfected with Endothelial Lipase / LIPG plasmid (Right) or empty vector (Left) for 48 hrs using GTX84191 Endothelial Lipase / LIPG antibody [4A8].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

Endothelial Lipase / LIPG antibody [4A8]

Cat. No. GTX84191

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84191 FACS Image

GTX84191 IHC-P Image

GTX84191 WB Image

GTX84191 IHC-P Image

GTX84191 FACS Image

GTX84191 IHC-P Image

GTX84191 IHC-P Image

GTX84191 IHC-P Image

GTX84191 IHC-P Image

GTX84191 FACS Image

GTX84191 ICC/IF Image

GTX84191 IP Image

GTX84191 IHC-P Image

GTX84191 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.