Endothelin 1 antibody [TR.ET.48.5]

Cat. No. GTX22786

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	TR.ET.48.5
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, RIA
Reactivity	Human, Mouse, Rat, Sheep, Dog, Pig
Package	100 μl ($399)

See all Endothelin 1 products

View product citations for antibody GTX22786 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:100-1:1000
ICC/IF	1:200-1:1000
IHC-P	1:250
IHC-Fr	1:250
FACS	0.5 μg/test
RIA	1:25,000

Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Product Note

This antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides. Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Pure endothelin-1 conjugated to KLH.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_384849

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endothelin 1 , ARCND3 , ET1 , HDLCQ7 , PPET1 , QME

Cellular Localization

Secreted

Background

This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Gene ID: 1906 EDN1
UniProt: P05305 EDN1

Research Area

DATA IMAGES

GTX22786 WB Image

WB analysis of 25 ug of PC12 cell lysate using GTX22786 Endothelin 1 antibody [TR.ET.48.5].
Dilution : 1:500

GTX22786 FACS Image

FACS analysis of 3T3 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX22786 ICC/IF Image

ICC/IF analysis of PC12 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

GTX22786 FACS Image

FACS analysis of 293T cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX22786 ICC/IF Image

ICC/IF analysis of Hela cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

GTX22786 ICC/IF Image

ICC/IF analysis of HUVEC cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

GTX22786 FACS Image

FACS analysis of HepG2 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

REFERENCE

Application Reference

Dimitrijevic I et al. Ophthalmology 2010; 117 (3) : 628-36 Increased tissue endothelin-1 and endothelin-B receptor expression in temporal arteries from patients with giant cell arteritis.

Application : IHC-P

Reactivity : Human

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REVIEW

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Application Reference

Dimitrijevic I et al. Ophthalmology 2010; 117 (3):628-36 Increased tissue endothelin-1 and endothelin-B receptor expression in temporal arteries from patients with giant cell arteritis.

Application : IHC-P

Reactivity : Human

SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

Endothelin 1 antibody [TR.ET.48.5]

Cat. No. GTX22786

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX22786 WB Image

GTX22786 FACS Image

GTX22786 ICC/IF Image

GTX22786 FACS Image

GTX22786 ICC/IF Image

GTX22786 ICC/IF Image

GTX22786 FACS Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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