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Endothelin A Receptor antibody

Anti-Endothelin A Receptor antibody used in Flow cytometry (FACS). GTX17405
Anti-Endothelin A Receptor antibody used in Western Blot (WB). GTX17405
Anti-Endothelin A Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17405

Cat. No. GTX17405

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
400 μl ($399)
See all Endothelin A Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human EDNRA.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endothelin receptor type A , ET-A , ETA , ETA-R , ETAR , ETRA , MFDA , hET-AR

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-Endothelin A Receptor antibody used in Flow cytometry (FACS). GTX17405

GTX17405 FACS Image

FACS analysis of CEM cells using GTX17405 Endothelin A Receptor antibody.
Green : EDNRA
Blue : Isotype control

Anti-Endothelin A Receptor antibody used in Western Blot (WB). GTX17405

GTX17405 WB Image

WB analysis of CEM cell lysate (35ug/lane) using GTX17405 Endothelin A Receptor antibody.

Anti-Endothelin A Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17405

GTX17405 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX17405 Endothelin A Receptor antibody.
Green : Endothelin A Receptor
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399