GeneTex
United States (US)

Endothelin B Receptor antibody [N1N2], N-term

GTX100356_39603_IHC_19011506_738.jpg
GTX100356_WB_19011506_358.jpg

Cat No. GTX100356

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

1XPBS, 50% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ETBR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Endothelin Receptor Type B,Abcds,Et-B,Et-Br,Etb,Etb1,Etbr,Etrb,Hscr,Hscr2,Ws4A,Ednrb

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Three transcript variants encoding two different isoforms have been found for this gene. While both isoforms bind ET1, they exhibit different responses upon binding, suggesting that they may be functionally distinct. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100356_39603_IHC_19011506_738.jpg

GTX100356 IHC-P Image

ETBR antibody [N1N2], N-term detects ETBR protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: ETBR antibody [N1N2], N-term (GTX100356) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100356_WB_19011506_358.jpg

GTX100356 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX100356 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169