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Endothelin B Receptor antibody

Anti-Endothelin B Receptor antibody used in Western Blot (WB). GTX100357
Anti-Endothelin B Receptor antibody used in Western Blot (WB). GTX100357
Anti-Endothelin B Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100357

Cat No. GTX100357

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Endothelin B Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Mouse brain , Rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 50% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of rat Endothelin B Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endothelin receptor type B , Ednra , Etb

Cellular Localization

Cell membrane

Background

Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Database

Research Area

DATA IMAGES

Anti-Endothelin B Receptor antibody used in Western Blot (WB). GTX100357

GTX100357 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX100357 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Endothelin B Receptor antibody used in Western Blot (WB). GTX100357

GTX100357 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
10% SDS PAGE
GTX100357 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Endothelin B Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100357

GTX100357 IHC-P Image

Endothelin B Receptor antibody detects Ednrb protein at membrane on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Endothelin B Receptor antibody (GTX100357) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Yuki Kai et al. JPS 2019; (Epub) Role of Rac1 in augmented endothelin-1-induced bronchial contraction in airway hyperresponsive mice.
Application : WB
Reactivity : Mouse
Lobna M. Abdel Moneim et al. Pharmacological Reports 2019; (Epub) Co-targeting of endothelin-A and vitamin D receptors: a novel strategy to ameliorate cisplatin-induced nephrotoxicity.
Application : IHC-P
Reactivity : Rat
Wang R et al. Environ Sci Pollut Res Int 2018; (Epub) Up-regulation of contractile endothelin receptors by airborne fine particulate matter in rat mesenteric arteries via activation of MAPK pathway.
Application : WB
Reactivity : Rat
Albano-Aluquin S et al. Am J Clin Exp Immunol 2017; 6 (6) : 107-114 An immunohistochemical analysis of folate receptor beta expression and distribution in giant cell arteritis - a pilot study.
Application : IHC-P
Reactivity : Human
Xiao X et al. Toxicol Sci 2016; 149 (1) : 213-26 The Role of MAPK Pathways in Airborne Fine Particulate Matter-Induced Upregulation of Endothelin Receptors in Rat Basilar Arteries.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169