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Enterovirus 71 2B antibody

Anti-Enterovirus 71 2B antibody used in Western Blot (WB). GTX132343
Anti-Enterovirus 71 2B antibody used in IHC (Paraffin sections) (IHC-P). GTX132343

Cat. No. GTX132343

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Enterovirus 71
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus 71 2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

EV71 C2 infected RD

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Enterovirus 71 2B (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886620

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Enterovirus 71 Protein 2B , EV71 Protein 2B

Research Area

DATA IMAGES

Anti-Enterovirus 71 2B antibody used in Western Blot (WB). GTX132343

GTX132343 WB Image

EV71 2B antibody detects EV71 2B protein by western blot analysis. Un-infected (-) and infected with EV71 C2 strain RD whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with EV71 2B antibody (GTX132343) at a dilution of 1:1000.

Anti-Enterovirus 71 2B antibody used in IHC (Paraffin sections) (IHC-P). GTX132343

GTX132343 IHC-P Image

EV71 2B antibody detects EV71 2B protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 2B antibody [GTX132343] diluted at 1:500
Antigen Retrieval: Citrate buffer

REFERENCE

Application Reference

Zhang X et al. Mol Ther 2020; 28 (6) : 1533-1546 Enterovirus A71 Oncolysis of Malignant Gliomas.
Application : IHC-P
Yen MH et al. Kaohsiung J Med Sci. 2017; (Epub) Artemisia capillaris inhibited enterovirus 71-induced cell injury by preventing viral internalization.
Application : WB
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REVIEW

Enterovirus 71 2B antibody Cat. No. GTX132343

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Enterovirus 71 2B antibody used in Western Blot (WB). GTX132343
Date : Anonymous submitted on 10-Sep-2021
Score :
Application Tested : WB
Sample Species : EV71
Sample : RD (ATCC CCL-136)
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : The RD cells were transfected with different amounts of pEGFP-N1-EV71-2B using Lipofectamine3000, the cell was harvested at 24h post-transfection.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169