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Enterovirus 71 3AB antibody

Anti-Enterovirus 71 3AB antibody used in Western Blot (WB). GTX132344
Anti-Enterovirus 71 3AB antibody used in IHC (Paraffin sections) (IHC-P). GTX132344

Cat. No. GTX132344

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Enterovirus 71
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus 71 3AB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

EV71 C2 infected RD , EV71 B4 infected RD , CVA16 infected RD

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

Synthetic peptide of Enterovirus 71 3AB (EV71 virus (strain USA/BrCr/1970)).

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886621

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Enterovirus 71 Protein 3AB , EV71 Protein 3AB

Research Area

DATA IMAGES

Anti-Enterovirus 71 3AB antibody used in Western Blot (WB). GTX132344

GTX132344 WB Image

Non-infected (–) and infected RD whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Enterovirus 71 3AB antibody (GTX132344) diluted at 1:5000.

Anti-Enterovirus 71 3AB antibody used in IHC (Paraffin sections) (IHC-P). GTX132344

GTX132344 IHC-P Image

EV71 3AB antibody detects EV71 3AB protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 3AB antibody [GTX132344] diluted at 1:500
Antigen Retrieval: Citrate buffer

REFERENCE

Application Reference

Fu Y et al. PLoS Pathog 2023; 19 (1) : e1011090 Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism.
Application : WB
Zhao X et al. J Virol 2022; 96 (6) : e0011922 N-Acetyltransferase 8 Promotes Viral Replication by Increasing the Stability of Enterovirus 71 Nonstructural Proteins.
Yu J et al. Virulence 2022; 13 (1) : 241-257 Regulation of host factor γ-H2AX level and location by enterovirus A71 for viral replication.
Application : WB
Yen MH et al. Kaohsiung J Med Sci. 2017; (Epub) Artemisia capillaris inhibited enterovirus 71-induced cell injury by preventing viral internalization.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169