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Enterovirus 71 3AB antibody

Anti-Enterovirus 71 3AB antibody used in IHC (Paraffin sections) (IHC-P). GTX132344
Anti-Enterovirus 71 3AB antibody used in Western Blot (WB). GTX132344

Cat. No. GTX132344

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Enterovirus 71
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus 71 3AB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

EV71 C2 infected RD , EV71 B4 infected RD , CVA16 infected RD

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Enterovirus 71 3AB (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886621

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Enterovirus 71 Protein 3AB , EV71 Protein 3AB

Research Area

DATA IMAGES

Anti-Enterovirus 71 3AB antibody used in IHC (Paraffin sections) (IHC-P). GTX132344

GTX132344 IHC-P Image

EV71 3AB antibody detects EV71 3AB protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 3AB antibody [GTX132344] diluted at 1:500
Antigen Retrieval: Citrate buffer

Anti-Enterovirus 71 3AB antibody used in Western Blot (WB). GTX132344

GTX132344 WB Image

Non-infected (–) and infected RD whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Enterovirus 71 3AB antibody (GTX132344) diluted at 1:5000.

REFERENCE

Application Reference

Zhao X et al. J Virol 2022; 96 (6) : e0011922 N-Acetyltransferase 8 Promotes Viral Replication by Increasing the Stability of Enterovirus 71 Nonstructural Proteins.
Yu J et al. Virulence 2022; 13 (1) : 241-257 Regulation of host factor γ-H2AX level and location by enterovirus A71 for viral replication.
Application : WB
Yen MH et al. Kaohsiung J Med Sci. 2017; (Epub) Artemisia capillaris inhibited enterovirus 71-induced cell injury by preventing viral internalization.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169