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Enterovirus 71 3C antibody [B3]

Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191
Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191

Cat No. GTX630191

Host

Mouse

Clonality

Monoclonal

Clone Name

B3

Isotype

IgG1

Application

WB, FACS

Reactivity

Coxsackievirus A6, Enterovirus 71
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
FACS Assay dependent
Not tested in other applications.

Positive Control

EV71-infected RD , EV71 C2-infected RD , CVA6-infected RD

Product Note

This antibody is raised against Enterovirus 71, and it may cross react with Coxsackievirus A6 based on internal testing.

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

The immunogen used to generate this antibody corresponds to EV71 3C

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888195

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Enterovirus 71 Protease 3C , Enterovirus 71 3C , EV71 Protease 3C , EV71 3C

Research Area

DATA IMAGES

Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191

GTX630191 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 3C antibody [B3] (GTX630191) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191

GTX630191 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 3C antibody [B3] (GTX630191) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Enterovirus 71 3C antibody [B3] Cat No. GTX630191

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31235851
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015. PMID: 26681405
SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169