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Enterovirus 71 VP1 antibody [GT185]

Orthogonal
Anti-Enterovirus 71 VP1 antibody [GT185] used in Western Blot (WB). GTX633390
Anti-Enterovirus 71 VP1 antibody [GT185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633390
Anti-Enterovirus 71 VP1 antibody [GT185] used in Western Blot (WB). GTX633390
Anti-Enterovirus 71 VP1 antibody [GT185] used in ELISA (ELISA). GTX633390

Cat. No. GTX633390

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT185

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Enterovirus 71
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus 71 VP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Product Note

This antibody was raised against Enterovirus 71 VP1, and it does not cross-react with Enterovirus D68 VP1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Enterovirus 71 VP1 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888339

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Enterovirus 71 Capsid protein VP1 , Enterovirus 71 VP1 , EV71 Capsid protein VP1 , EV71 VP1

Research Area

DATA IMAGES

Anti-Enterovirus 71 VP1 antibody [GT185] used in Western Blot (WB). GTX633390

GTX633390 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT185] (GTX633390) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody [GT185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633390

GTX633390 ICC/IF Image

Enterovirus 71 VP1 antibody [GT185] detects Enterovirus 71 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus 71 VP1 stained by Enterovirus 71 VP1 antibody [GT185] (GTX633390) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Enterovirus 71 VP1 antibody [GT185] used in Western Blot (WB). GTX633390

GTX633390 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT185] (GTX633390) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody [GT185] used in ELISA (ELISA). GTX633390

GTX633390 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Enterovirus 71 VP1 protein, His tag (GTX138560-pro) (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [GT185] (GTX633390) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

REFERENCE

Application Reference

L Liu et al. Virol Sin 2024; S1995-820X (24) : 00070-1 NSUN2 mediates distinct pathways to regulate enterovirus 71 replication
Wang Y et al. J Med Virol 2023; 95 (1) : e28400 Phosphorylation of enteroviral 2A(pro) at Ser/Thr125 benefits its proteolytic activity and viral pathogenesis.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169