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Enterovirus 71 VP1 antibody [HL1929]

Anti-Enterovirus 71 VP1 antibody [HL1929] used in ELISA (ELISA). GTX637688
Anti-Enterovirus 71 VP1 antibody [HL1929] used in ELISA (ELISA). GTX637688
Anti-Enterovirus 71 VP1 antibody [HL1929] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637688
Anti-Enterovirus 71 VP1 antibody [HL1929] used in Western Blot (WB). GTX637688
Anti-Enterovirus 71 VP1 antibody [HL1929] used in Western Blot (WB). GTX637688
Anti-Enterovirus 71 VP1 antibody [HL1929] used in Western Blot (WB). GTX637688

Cat. No. GTX637688

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1929

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

Enterovirus, Enterovirus 71
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX637688, Detection : GTX637687)
Please notice that GTX637687 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637688. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody was raised against Enterovirus 71 VP1, and it does not cross-react with Enterovirus D68 VP1 or Coxsackievirus A6.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

Synthetic peptide of Enterovirus 71 VP1. (strain USA/BrCr/1970)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Enterovirus 71 VP1 antibody [HL1929] used in ELISA (ELISA). GTX637688

GTX637688 ELISA Image

Sandwich ELISA detection of recombinant Enterovirus 71 VP1 protein, His tag (GTX138560-pro) using antibodies as below.

Capture: Enterovirus 71 VP1 antibody [HL1929] (GTX637688) (5 μg/mL)
Detection: HRP-conjugated Enterovirus 71 VP1 antibody [HL1928] (GTX637687) (1 μg/mL)
Please notice that GTX637687 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637688. Please contact us for custom HRP-conjugated antibody.

Anti-Enterovirus 71 VP1 antibody [HL1929] used in ELISA (ELISA). GTX637688

GTX637688 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Enterovirus 71 VP1 protein, His tag (GTX138560-pro) (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-Enterovirus 71 VP1 antibody [HL1929] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637688

GTX637688 ICC/IF Image

Enterovirus 71 VP1 antibody [HL1929] detects Enterovirus 71 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus 71 VP1 stained by Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Enterovirus 71 VP1 antibody [HL1929] used in Western Blot (WB). GTX637688

GTX637688 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody [HL1929] used in Western Blot (WB). GTX637688

GTX637688 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody [HL1929] used in Western Blot (WB). GTX637688

GTX637688 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations