Enterovirus 71 VP1 antibody [HL1929]

Sandwich ELISA detection of recombinant E.coli expressed, full-length Enterovirus 71 VP1 protein (GTX138560-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody [HL4796] (GTX644808)
Pair 2:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody [HL1929] (GTX637688)

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length Enterovirus 71 VP1 protein, His tag (GTX138560-pro) (1.79-114.29 nM). Coated protein was probed with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Sandwich ELISA detection of recombinant E.coli expressed, full-length Enterovirus 71 VP1 (GTX138560-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Enterovirus 71 VP1 antibody [HL1929] (GTX637688)
Detection: Enterovirus 71 VP1 antibody [HL4796] (GTX644808) conjugated with HRP
Pair 2:
Capture: Enterovirus 71 VP1 antibody [HL1928] (GTX637687)
Detection: Enterovirus 71 VP1 antibody [HL4796] (GTX644808) conjugated with HRP
Pair 3:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody [HL4796] (GTX644808) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant Enterovirus 71 VP1 protein, His tag (GTX138560-pro) using antibodies as below.

Capture: Enterovirus 71 VP1 antibody [HL1929] (GTX637688) (5 μg/mL)
Detection: HRP-conjugated Enterovirus 71 VP1 antibody [HL1928] (GTX637687) (1 μg/mL)
Please notice that GTX637687 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637688. Please contact us for custom HRP-conjugated antibody.

Enterovirus 71 VP1 antibody [HL1929] detects Enterovirus 71 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus 71 VP1 stained by Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-infected (–) and infected (+) RD whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) RD whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (GTX637688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.