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Enterovirus 71 VP1 antibody

Orthogonal
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Anti-Enterovirus 71 VP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132338
Anti-Enterovirus 71 VP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132338
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338

Cat. No. GTX132338

Reference 13
13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Enterovirus 71
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus 71 VP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

EV71 C2 infected RD , EV71 B4 infected RD , CVA16 infected RD

Product Note

This antibody was raised against Enterovirus 71 VP1, and it does not cross-react with Enterovirus D68 VP1.

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Enterovirus 71 VP1 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886616

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Enterovirus 71 Capsid protein VP1 , Enterovirus 71 VP1 , EV71 Capsid protein VP1 , EV71 VP1

Research Area

DATA IMAGES

Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338

GTX132338 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132338) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132338

GTX132338 ICC/IF Image

EV71 VP1 antibody detects EV71 VP1 protein by immunofluorescent analysis. Sample: RD cells infected with EV71 were fixed in 4% paraformaldehyde. Green: EV71 VP1 protein stained by EV71 VP1 antibody (GTX132338) diluted at 1:100. Blue: DAPI staining. Scale bar = 500 μm.

Anti-Enterovirus 71 VP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132338

GTX132338 IHC-P Image

EV71 VP1 antibody detects EV71 VP1 protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 VP1 antibody [GTX132338] diluted at 1:500
Antigen Retrieval: Citrate buffer

Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338

GTX132338 WB Image

Non-infected (–) and infected RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132338) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338

GTX132338 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132338) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Fu Y et al. PLoS Pathog 2023; 19 (1) : e1011090 Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism.
Cui G et al. Front Pharmacol 2022; 13 : 1027566 Berberine prevents lethal EV71 neurological infection in newborn mice.
Hsieh CF et al. J Virol 2022; 96 (7) : e0054221 3,4-Dicaffeoylquinic Acid from the Medicinal Plant <i>Ilex kaushue</i> Disrupts the Interaction Between the Five-Fold Axis of Enterovirus A-71 and the Heparan Sulfate Receptor.
Xu M et al. PLoS Pathog 2022; 18 (9) : e1010794 Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection.
Zhao X et al. J Virol 2022; 96 (6) : e0011922 N-Acetyltransferase 8 Promotes Viral Replication by Increasing the Stability of Enterovirus 71 Nonstructural Proteins.
Show More +
Submit a Reference

REVIEW

Enterovirus 71 VP1 antibody Cat. No. GTX132338

Rating
( Average 5 based on 13 users reviews)
Application
Western Blot(WB)
( Average 5 based on 11 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019. PMID: 31575039
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018. PMID: 29922292
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018. PMID: 29922292
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019. PMID: 31575039
Anti-Enterovirus 71 VP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018. PMID: 29922292
Anti-Enterovirus 71 VP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018. PMID: 29922292
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019. PMID: 31575039
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018. PMID: 29922292
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018. PMID: 29922292
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018. PMID: 29922292
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018. PMID: 29922292
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019. PMID: 31575039
Anti-Enterovirus 71 VP1 antibody used in Western Blot (WB). GTX132338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : EV71
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018. PMID: 29922292
Show More +
Submit a Review
Application Reference
Fu Y et al. PLoS Pathog 2023; 19 (1):e1011090 Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism.
Cui G et al. Front Pharmacol 2022; 13:1027566 Berberine prevents lethal EV71 neurological infection in newborn mice.
Hsieh CF et al. J Virol 2022; 96 (7):e0054221 3,4-Dicaffeoylquinic Acid from the Medicinal Plant <i>Ilex kaushue</i> Disrupts the Interaction Between the Five-Fold Axis of Enterovirus A-71 and the Heparan Sulfate Receptor.
Xu M et al. PLoS Pathog 2022; 18 (9):e1010794 Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection.
Zhao X et al. J Virol 2022; 96 (6):e0011922 N-Acetyltransferase 8 Promotes Viral Replication by Increasing the Stability of Enterovirus 71 Nonstructural Proteins.
Yuanmin Dong et al. Front Microbiol 2021; 12:806084 Enterovirus 71 Antagonizes Antiviral Effects of Type III Interferon and Evades the Clearance of Intestinal Intraepithelial Lymphocytes.
Application : ICC/IF
Han Fu et al. Am J Transl Res 2020; 12 (12):8247-8255 Brequinar inhibits enterovirus replication by targeting biosynthesis pathway of pyrimidines.
Application : WB, ICC/IF
Du M et al. Anal Chem 2019; (Epub) Target-Induced Cascade Amplification for Homogeneous Virus Detection.
Guo H et al. Nat Commun 2019; 10 (1):4066 A second open reading frame in human enterovirus determines viral replication in intestinal epithelial cells.
Application : WB
Wang H et al. Int J Mol Sci 2019; 20 (19) STAT3 Regulates the Type I IFN-Mediated Antiviral Response by Interfering with the Nuclear Entry of STAT1.
Application : WB
Han Kang Tee et al. bioRxiv 2019; (Epub) Electrostatic interactions at the five-fold axis alter heparin-binding phenotype and drive EV-A71 virulence in mice.
Application : IHC-P
Chen D et al. Front Immunol 2018; 9:1268 Promyelocytic Leukemia Restricts Enterovirus 71 Replication by Inhibiting Autophagy.
Chen D et al. Int Immunopharmacol 2018; 60:111-120 Harmine, a small molecule derived from natural sources, inhibits enterovirus 71 replication by targeting NF-κB pathway.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169