Enterovirus 71 VP1 antibody

Sandwich ELISA detection of recombinant E.coli expressed, full-length Enterovirus 71 VP1 protein (GTX138560-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody (GTX132338)
Pair 2:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody (GTX132339)
Pair 3:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody (GTX132600)

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132339) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132339) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EV71 VP1 antibody detects EV71 VP1 protein by immunofluorescent analysis. Sample: RD cell infected with EV71 were fixed in 4% paraformaldehyde. Green: EV71 VP1 protein stained by EV71 VP1 antibody (GTX132339) diluted at 1:100. Blue: DAPI staining. Scale bar = 500 μm.

Non-infected (–) and infected RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132339) diluted at 1:5000.

EV71 VP1 antibody detects EV71 VP1 protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 VP1 antibody [GTX132339] diluted at 1:500
Antigen Retrieval: Citrate buffer

The data was published in the journal Viruses in 2020.PMID: 32276428

The data was published in the journal Viruses in 2020.PMID: 32276428

The data was published in the journal Viruses in 2020.PMID: 32276428